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Multivariate analysis revealed that the co-expression of SOX2 with HR-HPV RISH may be an independent factor of OS [hazard ratio = 3.597; 95% confidence interval (CI) 1.085-11.928; P = 0.036] and DFS [hazard ratio = 2.880; 95% CI 1.199-6.919; P = 0.018] prediction in SCNEC. Overall, the results of the present study suggest that the co-expression of SOX2 with HR-HPV RISH in SCNEC may represent a specific subgroup exhibiting remarkably poorer prognostic outcomes compared with the expression of any one marker alone. Overall, the results of