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Thus, to partly alleviate the noise in expression estimates, they propose a new normalization method called length-rescaled CPM. Remarkably, the authors found considerable distortions at the nucleotide level, which leads to an underestimation of diversity in transcriptome assemblies. The study by Freedman et al. (Mol Ecol Resourc 202 clearly shows that we have not yet reached "high-quality" in the field of transcriptome assembly. Above all, it helps researchers be aware of these problems and filter and interpret their transcriptome as
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