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Hypothalamic amenorrhea is common in adolescents and young adults (AYAs) with anorexia nervosa (AN), and ovarian reserve is not routinely assessed. AN increases rates of fertility problems, but how or when AN negatively influences future fertility is unclear. We sought to determine whether biomarkers of ovarian reserve were impacted in AYA with AN. Cross-sectional study. Tertiary care center. Females with AN and amenorrhea (n=97) at the pre-intervention visit of a clinical trial, n=19 females without an eating disorde