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More over, six regarding the 19 loci amplified when tested into the congeneric types T. pusilla. Conclusions The 19 recently developed microsatellite markers can be used to explain hereditary diversity and population structure of communities of T. calyculata. © 2020 Vlasta et al. Programs in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.An emerging approach to ecosystem monitoring requires the use of physi