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A kind of biomaterial with antibacterial and mechanical properties was prepared using gelatin (GE) as a raw material. GE was modified by antibacterial epoxy quaternary ammonium salt (QAS) and then cross-linked with tannic acid (TA). Analysis of the Fourier transform infrared spectroscopy (FTIR) results showed that the cationic group was grafted onto GE by reaction of the amino of GE with the epoxy of QAS, and the cross-linking occurred between the amino of GE and the active groups of TA under alkaline conditions. The cross-linki