https://s1preceptor.com/index.....php/early-on-detecti
At each and every time point, sub-regions of an mRNA are spectrally distinguished to probe transcription elongation and early cancellation. The outcome of this protocol additionally enables analyzing intracellular localization of mRNAs and heterogeneity in mRNA copy numbers among cells. Utilizing this protocol many samples (~5 may be prepared within 8 h, like the length of time necessary for just a couple of samples. We discuss how to apply this protocol to examine the transcription and degradatio
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