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Mild MDD was clinically diagnosed in 23%, moderate MDD in 12%, and severe MDD in 14% of the participants. The IRT method identified the following HADS depression score thresholds for mild, moderate, and severe MDD 10.7, 13.2, and 15.1, respectively. Conclusion The new IRT method identifies clinical thresholds that are unbiased by disease prevalence.Emerging evidence suggests that the gut microbiome plays an important role in the pathophysiology of both obesity and type 2 diabetes mellitus. We previously reported that dietary annatto-extr
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