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In the long run, this technique may raise some moral issues, corresponding to overpopulation, leading to decrease availability of meals and vitality. NutriStem with LN-511, TeSR2 with human recombinant laminin (LN-511), and RegES with human foreskin fibroblasts (HFFs) are commonly used xeno-free tradition techniques [33]. There are many organizations and international initiatives, such as the National Stem Cell Bank, that provide stem cell lines for treatment or medical research [77]. Viral testing—When