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Infections with nontuberculous mycobacteria (NTM) have a poor prognosis in patients with underlying respiratory diseases. Clofazimine (CFZ) showed both experimental and clinical promising results against clinically relevant NTM. However there is no data on CFZ in combination with the current recommended treatment therefore we aimed to study its in vivo activity in an aerosol mouse model of M. aviumIn an aerosol infection BALB/c mouse model using M. avium strain Chester we treated 58 mice with four combinations of rifampicin (RIF) 10mg