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While transcriptional alterations in hypoxic cardiomyocytes are well-studied, little is known in regards to the proteins being earnestly released from these cells. Practices We established a novel secretome analysis of cardiomyocytes by combining stable isotope labeling and click chemistry with subsequent size spectrometry evaluation. More functional validation experiments included ELISA measurement of human samples, murine LAD ligation and adeno-associated virus (AAV) 9-m