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ocol 4 Growth and maintenance of human A2EN cells in monolayer culture Support Protocol 5 Preparation of the slow-turning lateral vessel bioreactor Support Protocol 6 Preparation of Cytodex-3 microcarrier beads Basic Protocol 3 Histological assessment of 3D organotypic raft tissues Basic Protocol 4 Spatial analysis of protein expression in 3D organotypic raft cultures Basic Protocol 5 Immunofluorescence imaging of RWV-derived 3D tissues Basic Protocol 6 Ultrastructural visualization and imaging of RWV-derived 3D tissues Basic Protoc