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This first case report of pediatric M. mageritense infection highlights an important potential pitfall of MALDI-TOF MS. We also report that the choice of antimicrobials for treatment of M. mageritense may be more limited in children than that for adults.Several outbreaks of trench fever caused by Bartonella quintana, occurred in soldiers during World Wars I and II. Although the number of trench fever cases has been decreasing worldwide, the disease has been reported among the homeless population in both developing and developed coun