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In this paper dispersive liquid-liquid microextraction using deep eutectic solvent (DES), as an extraction solvent, was applied for the pre-concentration and determination of parabens in liquid pharmaceutical samples. A DES composed of a hydrogen bond acceptor [choline chloride (ChCl)] and a hydrogen bond donor (glucose) achieved the highest extraction efficiency. Therefore, this solvent was selected as the extraction solvent. After the synthesis of this solvent, its various properties were investigated. Thermogravimetric analysis, X-r