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Polymer electrolyte membrane unitized regenerative fuel cells (PEM-URFCs) require bifunctional porous transport layers (PTLs) to play contradictory roles in a single unitized system hydrophobicity for water drainage in the fuel cell (FC) mode and hydrophilicity for water supplement in the electrolysis cell (EC) mode. Here, we report a high-performance amphiphilic Ti PTL suitable for both FC and EC modes, thanks to alternating hydrophobic and hydrophilic channels. To fabricate the amphiphilic PTL, we used a shadow mask patterning process