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Strengthen Muscles With Stationary Bike Exercise You can still get a great workout on a stationary bicycle even if you don't want or aren't able to join a cycling class at the local gym. This kind of exercise burns calories, strengthens muscles and can even aid in relieving arthritis symptoms. The hip flexor is one of the major muscles that is worked in a cycling workout. The muscle contracts during the second part of your pedal stroke, bringing your straight leg into a flexed posture.