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L-1 and urea concentration of 0.3 g.dL-1 were found optimum conditions with a predicted glutamate production of 19.84 mg.mL-1 with desirable level 1. Therefore RSM can be an effective method to optimize glutamate production and the findings of this study are a guideline for the other amino acids fermentation by C. glutamicum.Central venous catheterization (CVC) remains a common practice in the emergency setting. Routine flushing 10-20 ml of normal saline to maintain the patency of CVC could affect the accuracy of laboratory tests. Typic
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