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In Stage 3, release of Hg in 10%MOAKs was eight-fold lower than in soil controls (SedCTRs), while increases in aqueous (up to 21 ng L-1) and solid (up to 88 ng g-1) MeHg concentrations were observed. The increases in MeHg corresponded to elevated aqueous concentrations of Mn, Fe, SO42-, and HS- in Stage 3. Results of S K-edge X-ray absorption near edge structure (XANES) analysis suggest oxidation of S in Stage 2 and formation of polysulfur in Stage 3. Results of pyrosequencing analysis indicate sulfate-reducing bacteria (SR became ab