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The statistical methods (Plakett-Burman and reaction area methodology) were more useful for the optimization of medium elements. Medium composition 0.1% w/v sugar, 0.3% w/v yeast herb, 0.03% w/v magnesium sulphate, 0.20% w/v potassium dihydrogen phosphate and 2.5% w/v salt chloride with inoculum size (1% v/v) ended up being suitable for greater GGT titres (449 U ml-1). Time kinetics revealed the security of enzyme up to 96 h of incubation suggesting its application in the professional u