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miR-335 was significantly lower in NSCLC samples compared to non-cancerous samples (P less then .001). of miR-335 was significantly correlated with tumor histology (P = .028), lymph node metastasis (P = .002), differentiation degree (P less then .001), and pathological TNM stage (P less then .001). The log-rank test indicated that patients with decreased miR-335 expression experienced poor overall survival in NSCLC (P = .029).The results of the present study indicated that miR-335 was down-expressed in NSCLC, and is associated with