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By using this ability, we monitored the trajectories of an individual nanoparticle getting together with a surface, sized the hydrodynamically hindered diffusion of nanoparticles, and disclosed the surface chemistry-dependent behavior of nanoparticles at the program. The application form for monitoring formation of membranes from a lipid vesicle had been shown, suggesting the potential for investigating an extensive number of nano-objects at interfaces in a complex