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5 ± 0.04 for syringic acid and 0.6 ± 0.06 for vanillic acid. The results obtained in the study of anti-oxidant activity of methanol extract R. communis(MERC) and E. hirta (MERH) were showed significant free radical scavenging capacity against DPPH generated free radicals. The developed HPTLC method was validated for accuracy, linearity, precision, and specificity. Both the extract has revealed considerable antioxidant activity. The reported existing phenolic and flavonoids compounds are responsible for antioxidant activity of plant extr