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Aim To determine the prevalence and factors associated with hepatitis B virus infection among pregnant women attending antenatal clinic in Mulago Hospital. Design Cross-sectional observational study. Setting Mulago National Referral Hospital, Uganda, antenatal clinic. Participants We randomly selected 340 pregnant women attending their first antenatal visit at Mulago Hospital antenatal clinic. Primary outcome Hepatitis B surface antigen positivity. Results We recruited 340 participants, with a mean age of 27±5.7 years, and a median gra