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In addition the new method gives a quantifiable level of confidence in each classification. AVAILABILITY The software can be found at https//github.com/bioarch-sjh/bacollite, and can be installed in R using devtools. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online. © The Author(s) 2020. Published by Oxford University Press.MOTIVATION Single-cell RNA-seq makes possible the investigation of variability in gene expression among cells, and dependence of variation on cell type. Statistical inference method