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Initially, obtainment of artichoke POS by Pectinex® Ultra-Olio ended up being optimised utilizing an artificial neural system. Under ideal conditions (pH 6.86; 1.5 h; enzyme dose 520.5 U/g pectin) POS yield was 624 mg/g pectin. Oligosaccharide structures (Mw less then 1.3 kDa) were characterised by MALDI-TOF-MS. Then, conformational evaluation of glycosidic bonds was carried out by replica trade molecular characteristics simulations and communication m