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Anti-inflammatory activity of substances 5 and 7-17 were assessed with real human neutrophils as a result towards the stimulation of formyl-methionyl-leucyl phenylalanine (fMLP). Asperlactone (9), aspyrone (13), and (-)-(3R)-mellein (14) exerted superoxide anion inhibition at 30 ± 9%, 29 ± 9%, and 26 ± 12%, respectively, at 10 μM. The capacities of asperlactone (9), aspilactonol B (1, penicillic acid (12), and (-)-(3R)-mellein (14) in elastase release inhibition had been revealed as 25 ± 4%, 3