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This research had been performed in Central Ethiopia to determine the prevalence and genotypes of E. bieneusi in lambs to be able to assess their general public health value. 3 hundred eighty nine fecal samples were collected and screened utilizing a nested PCR focusing on the inner transcribed spacer (ITS) for the ribosomal RNA gene. All positive PCR products were sequenced to determine the genotypes. E. bieneusi was found in 39 (10.03%) associated with the lambs. Differ