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This method may be used as a nonlinear nanofocusing light source to increase the light-matter nonlinear interaction.BACKGROUND DHA can regulate various physiological functions of cells. Our group has clarified the immunomodulatory activity and molecular mechanism of DHA on RAW264.7 cells. However, the effect of DHA on the membrane fatty acid environment and the activation of signaling pathways on the cell membrane is still not clear. METHODS In this study, we evaluated the fluidity, the potential and the fatty acid, phospholipid and pr