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The second-order rate constants for IMI degradation with OH• were calculated as 2.23 × 1011 and 9.08 × 1011 M-1 s-1 for the O3 alone and O3/UV procedures, correspondingly. The IMI degradation path analysis showed that IMI destroyed NO2, HNO2, after which Cl- from its structure, and also the O3/UV process yielded less by-products than O3.Laccases are a group of copper-containing oxidase enzymes present in aquatic and terrestrial environment. They