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Time-of-flight secondary ion mass spectrometry confirmed the introduction of amino groups, and solid-state 13C nuclear magnetic resonance spectra provided a support for inferring the position of the amino group. The relevance of subcortical structures for affective processing is not fully understood. Inspired by the gerbil retino-raphe pathway that has been shown to regulate affective behavior and previous human work showing that the pontine region is important for processing emotion, we asked whether well-established tracts in humans