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In this study, we investigated the result of enzymatically synthesized glycogen on ultraviolet B-induced oxidative stress and apoptosis in typical personal epidermal keratinocytes. Treatment with enzymatically synthesized glycogen suppressed ultraviolet B-induced reactive oxygen species, caspase-3 activity, and DNA fragmentation in regular personal epidermal keratinocytes. Furthermore, enzymatically synthesized glycogen increased into the phrase level of heme oxygenase-1, NAD(P)H