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Additionally, strong associations were found between plasma -glycans and lymph node metastasis in TC, which added the accuracy of predicting metastasis before surgery to the existing method. We comprehensively evaluated the plasma -glycomic changes in patients with TC or BTN for the first time. We determined several -glycan biomarkers, some of them have potential in the differential diagnosis of TC, and the others can help to stratify TC patients to low or high risk of lymph node metastasis. The findings enhanced the understanding of