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© 2020 Wiley-VCH Verlag GmbH Co. KGaA, Weinheim.OBJECTIVE To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitis patients and correlate them with clinical inflammatory periodontal parameters. MATERIAL AND METHOD An exploratory study, including 86 subjects was conducted. Clinical and periodontal data were recorded and oral fluids samples were obtained. EVs were precipitated by ExoQuick-TC™ and characterized by nanoparticle tracking analysis (Nanosi