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Among the genes deleted in NTV, C7L or/and K1L gene was mainly responsible for its replication defect. Protein C7 interacted with SAMD9, which antagonized the antiviral response of SAMD9 to ensure viral protein translation and replication of NTV in non-permissive cell lines. Our finding will serve as a baseline for modification of NTV in future application. Copyright © 2020 Zhao, Zhao, Huang, Ren, Zhang, Tian and Tan.Current molecular PCR-based techniques used for detecting Streptococcus pneumoniae, the causative pathogen of invasive pneu