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As two important reactive oxygen species, hydrogen peroxide (H2O2) and hypochlorous acid (HClO) play vital roles in many physiological and pathological processes. However, the relationship between these two species is seldom investigated, in part, because of the lack of robust molecular tools that can simultaneously visualize HClO and H2O2 in biosystems. In this work, we present a design strategy to construct a single fluorescent probe that can detect H2O2 and HClO by simultaneously monitoring two distinct detection channels. In the desi