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The desulfurization of fuel is currently enforced to meet environmental legislation and prevent pollution. The use of specific biodesulfurizing microbes with a unique 4S pathway allows the desulfurization without compromising the quality of fuel. These specific microbes can be screened by the detection of 2-hydroxybiphenol (2-HBP) in desulfurizing mixture of dibenzothiophene (DBT). At present, colorimetric Gibb's assay is the most commonly employed screening method which requires a specific reagent, i.e., 2,6-dichloroquninone-4-chlorami