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Eventually, we document the generation and antagonistic activity of a single-chain IL-4/13trap, which inhibits both IL-4 and IL-13 signaling. Collectively, these results illustrate that single-chain soluble receptor fusion proteins against IL-4, IL-13, and IL-33 are novel biologics which may not only be of great interest for research purposes and further interrogation associated with the role of their target cytokines in physiology and condition, but may also enhance monoclona