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In addition, patients' cohort alone was analyzed, (excluding the healthy controls), and the effect of acetazolamide treatment on their gut microbiota was analyzed. IIH patients have a lower diversity of bacterial species compared with healthy individuals. These bacteria, that is, Lactobacillus ruminis (L. ruminis) (p6.95E-08), Atopobium parvulum (p3.9E-03), Megamonas hypermegale (p5.61E-03), Ruminococcus gnavus (p1.29E-02), MEL.A1 (p3.04E-02), and Streptococcus sp. I-G2 (p3.04E-02), were previously characterized