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Aim To established a simple, controlled and reproducible method to synthesize gallium (Ga)-coated polydopamine (PDA) nanoparticles (NPs). Materials methods PDA NPs were synthesized in alkali medium with posterior Ga shell formation due to ion chelation on the NP surface. Results The obtained results with energy-dispersive x-ray spectroscopy confirmed the incorporation of Ga on the PDA NP surface. The cytotoxicity of Ga-coated PDA NPs was evaluated in vitro at different concentrations in contact with human adipose-derived stem cells. Further ce