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The effect of duration of optotype presentation on visual acuity measures has been extensively studied under photopic conditions. However, systematic data on duration dependence of acuity values under mesopic and scotopic conditions is scarce, despite being highly relevant for many visual tasks including night driving, and for clinical diagnostic applications. The present study aims to address this void. We measured Landolt C acuity under photopic (90cd/m ), mesopic (0.7cd/m ), and scotopic (0.009cd/m ) conditions for several optotype pre