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The combination with carbenoxolone significantly enhanced the inhibitory effect of RSL3 on colony formation, wound healing rate ( =0.005), invasion and migration of the cells ( 0.001). Fer-1 obviously attenuated the inhibitory effects of RSL3 alone and its combination with carbenoxolone on I-10/DDP cells ( 0.01). RSL3 treatment significantly decreased GPX4 expression ( =0.001) and increased lipid ROS level ( =0.001) and Fe level in the cells, and these effects were further enhanced by the combined treatment with carbenoxolone
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