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The simulation and phantom experiments results showed that the ATSB algorithm could reconstruct the target size more accurately and could distinguish multiple targets more effectively than the other three algorithms.Significance. The ATSB algorithm could improve the image quality of MIT and better meet the needs of clinical applications and is expected to promote brain injury monitoring imaging via MIT.Branched AuNPs usually have two or more local surface plasmon resonance (LSPR) absorption bands due to their structural anisotropy, and