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Since sampling size depends upon the amount of Landmarks (LMs) used, this research aimed to establish the minimal quantity of wing LMs needed to enhance GM analysis of mosquito types and/or genera from metropolitan and peri-urban areas of Argentina. Female left wings were used for the optimization period, in which 17 LMs were paid off to four by iterative LM exclusion. To confirm its efficiency, Principal Component review (PCA), Discriminant Analysis (DA), and Canonical Variate