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Overall, our observations indicate that sperm transcripts carrying chromatin imprints may play an important role in human development and cancer.Background The aortic valve (AV) is the most commonly affected valve in valvular heart diseases (VHDs). The objective of the study is to identify microRNA (miRNA) molecules expressed in VHDs and the differential expression patterns of miRNA in AVs with either calcification or rheumatism etiologies. Methods Human AVs were collected during valve replacement surgery. RNA was extracted and miRNA containing libraries we