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Molecular markers are usually more effective and precise in explaining the hereditary functions when it comes to identification and purity assessment of cultivars. In our research, we assessed the distinctness and uniformity of five soybean cultivars utilizing both solitary nucleotide polymorphism (SNP) markers produced by specific-locus increased fragment sequencing (SLAF-seq) technology, and easy series perform (SSR) markers. The phylogenetic tree and princip