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IL-1β (10 ng/ml) was used to determine a NP cells degenerated design. We analyzed the appearance of caspase 3, caspase 8, RIPK1, RIPK 3, and MLKL in different amount of degenerate disc tissues. Cell viability was examined because of the Cell Counting Kit-8 (CCK-8) assay. The phrase degrees of collagen Ⅱ, β-galactosidase (β-gal), caspase 3, caspase 8, RIPK1, RIPK 3, and MLKL, several inflammatory and anti-oxidant enzymes various NP cell treat groups were detected by

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