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Application of EM to fresh cattle manure, inoculated with ~5-6 log CFU g of antibiotic-resistant strain of indicator organism E. coli ATCC 25922, resulted in complete elimination of the organism in 20 days, while survivors were still detected in the untreated counterpart. EM can potentially be used for sustainable pathogen control in cattle manure for enhanced food safety and environmental health. © 2020 Society of Chemical Industry. EM can potentially be used for sustainable pathogen control in cattle manure for enhanced food safety and