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In this work, we created a SERS-active nanopipette you can use to do lasting and trustworthy intracellular analysis of single-living cells with minimal harm, which can be accomplished by optimizing the nanopipette geometry additionally the area density of this silver nanoparticle (AuNP) level at the nanopipette tip. To demonstrate its capability in single-cell analysis, we used the nanopipette for intracellular pH sensing. Intracellular pH (pHi) is key to cells because it affects mo

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