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In this research, an immobilized biofilm reactor was established for the simultaneous removal of calcium (Ca2+), fluoride (F-), nickel (Ni2+), and nitrate (NO3--N) by microbial induced calcium precipitation (MICP). The operating parameters of the reactor, hydraulic retention time (HRT 4, 8, and 12 h), influent Ca2+ concentration (36.0, 108.0, and 180.0 mg L-1), and influent Ni2+ concentration (0.0, 3.0, and 6.0 mg L-1) were discussed. Under the HRT of 12 h, influent Ca2+ concentration of 180.0 mg L-1, and influent Ni2+ concentration of 3