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the aim of this study was to analyze the chronobiology influence on the mechanical, kinematic, and physiological variables in a mountain bike (MT time trial. 16 mountain bike (MT male athletes volunteered to participate. Their characteristics were as follows body mass 70.2 ± 5.4 kg, stature 172.7 ± 4.0 cm, body fat 9.8 ± 3.5%, and VO 52.3 ± 3.9 mL/kg/min. Two 20 min MTB maximal protocols were applied, the first one in the morning and a second one in the afternoon period. No differences were found for all the variables studied, except