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Background Surgical assistance applications for smartphones have the potential to be used in daily practice, but regular reviews of these tools are required. StereoCheck® (Mevis®, São Paulo, Brazil) is a mobile app designed to compute stereotactic coordinates, as a checking tool. Objective Evaluate the accuracy and reliability of StereoCheck®. Methods This observational and prospective study involved 26 patients submitted to frame-based stereotactic brain biopsy. A standard stereotactic planning software (Framelink® 5.0, Medtronic®, Minn